Stable isotope probing

Stable isotope probing (SIP) is an isotope tracing technique that works by adding isotopically labeled compounds to environmental samples, and then identifying the functional microorganisms in environmental samples by molecular biological analysis of biomarkers (such as DNA, RNA or proteins), in which the biomarkers can provide taxonomic information and are labeled by stable isotopes. SIP has realized the purpose of directly combining the metabolic function and interaction of microorganisms with microbial population without separation and culture. It expands the utilization space of microbial resources and has broad application prospects.

Alfa Chemistry has global experimental and analysis abilities based on our comprehensive technical platforms and professional R&D team. We can provide you with rigorous SIP experimental design to ensure reliable analysis results.

Alfa Chemistry's Workflow

The SIP in Alfa Chemistry involves the following steps: Environmental samples are first exposed to a contaminant matrix with stable isotope labeling. Subsequently, the biomarkers (such as nucleic acids (DNA and RNA) and phospholipid fatty acids (PLFA) labeled by isotope are synthesized in the process of growth and reproduction of microorganisms by assimilated the labeled substrates. Finally, through the extraction, purification, isolation, identification and comparative analysis of biomarkers, the identification of functional microorganisms driving specific ecological processes in environmental samples [1]. The workflow diagram is as follows.

The workflow diagram of SIP.Fig. 1. The workflow diagram of SIP.

Alfa Chemistry's SIP

The SIP in Alfa Chemistry involves following three types:

  • Phospholipid derived fatty acid- stable isotope probing (PLFA-SIP)
  • Deoxyribonucleic acid-stable isotope probing (DNA-SIP)
  • Ribonucleic acid-stable isotope probing (RNA-SIP)

Alfa Chemistry' SIP

Alfa Chemistry's SIP Service Items

ItemsMain content
Study on biodegradation of organic pollutantsSIP technology can be used to study of biodegradation of benzene series (BTEX) (such as benzene, toluene, ethylbenzene and xylene), polycyclic aromatic hydrocarbons (PAHs) (such as naphthalene, phenanthrene, anthracene, pyrene), polychlorinated biphenyls (PCBs) and other organic pollutants, and then promoting the study of resource utilization of microbial remediation in organic pollutants and mechanism of microbial degradation.
Study on carbon cycling in rhizosphere microorganismsSIP technology is used to study plant-microbe-microfauna interactions around plant roots in soil.
Study on soil nitrogen cycleSIP technology is used to study the microbial functional groups and their interactions in soil nitrogen cycle.
Study on metagenomicsSIP technology is used to in-depth study of genetic diversity of difficult-to-culture microorganisms and its relationship to the environment.
Study on interbedded microorganismSIP technology is used to study the survival of one microorganism dependent on another in an anaerobic environment.

The above items are only part of the test items in Alfa Chemistry. Please contact us for more service information.

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  1. Wang J. and Yao H. Applications of DNA/RNA-stable isotope probing (SIP) in environmental microbiology[M]. Methods in Microbiology. Academic Press, 2021, 48: 227-267.
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