Covalent radiolabeling of biomacromolecules refers to a technique that involves attaching radioactive isotopes to biomacromolecule (such as protein) through covalent bonds. This labeling can be used to track and detect information about the distribution, function, and interactions of biomacromolecules within a biological organism. Take labeled proteins, for example, in this technique, specific radioactive isotopes (such as tritium, carbon-14, phosphorus-32, etc.) are typically selected to form covalent bonds with certain chemical groups of the protein (such as amino acid side chains or terminals). By this means, proteins can acquire radioactivity, allowing their application in fields such as biological imaging, drug development, and protein interaction studies. At Alfa Chemistry, our laboratory specializes in the covalent radiolabeling of biomacromolecules, offering end-to-end solutions tailored to specific research needs. We combine state-of-the-art facilities, advanced techniques, and a team of highly trained professionals to deliver radiolabeled biomacromolecules with high specific activity, excellent chemical purity, and reproducible quality.
Our Services
Alfa Chemistry employs isotopes tritium or carbon-14 to radiolabel large biomolecules. Key features of our service including:
- Compatibility with Diverse Biomolecules: Covalent radiolabeling is versatile and applicable to a wide array of macromolecules.
- Targeted Radiolabeling at Specific Sites: Our direct radiolabeling methods target specific functional groups within biomolecules for precision and stability. Common targets include lysine residues and N-terminal amino acids.
- Broad Molecular Weight Range: Our methods accommodate a wide molecular weight range of biomolecules, from 1 kDa to complex macromolecules exceeding 350 kDa. This flexibility allows us to handle everything from small drug candidates to large protein complexes and antibodies.
- Minimal Impact on Functionality: The low degree of chemical modification preserves the biomolecule's binding properties and biological activity, making it ideal for accurate experimental outcomes.
How Can Our Service Help You?
Our radiolabeling service provides valuable insights into the behavior of drugs and biomolecules in various research applications, including:
1. Pharmacokinetics Studies
Radiolabeled biomolecules enable precise tracking of drug absorption, distribution, metabolism, and excretion.
2. Quantitative Whole-Body Autoradiography (QWBA) and Microautoradiography (MARG)
These techniques utilize radiolabeled molecules to study the tissue distribution of drugs at microscopic and macroscopic levels.
3. Metabolic and Degradation Pathways
By tracing the radiolabeled biomolecule, researchers can identify metabolites and degradation products with accuracy.
4. Enhanced Drug Design
The insights gained from radiolabeling studies inform drug optimization, helping researchers design molecules with improved efficacy and reduced side effects.
QWBA: It provides quantitative data on the drug's localization in various organs and tissues, crucial for understanding therapeutic effects and potential toxicities.
MARG: It offers high-resolution imaging to examine drug distribution within specific tissues or even cellular compartments, enabling detailed histological analysis.
Why Partner with Us?
Expertise and Precision: Our experienced team specializes in radiolabeling, ensuring stable and accurate incorporation of tritium and carbon-14.
Customized Solutions: We tailor radiolabeling strategies to fit diverse research needs, including pharmacokinetics, tissue distribution, and metabolism studies.
Scalable and Efficient: From small-scale to large-scale projects, we deliver high-quality radiolabeled compounds quickly and efficiently.
Comprehensive Quality Control: We provide thorough analytical support, including purity, isotopic distribution, and stability testing, to guarantee reliable outcomes.
At Alfa Chemistry, our advanced techniques, tailored solutions, and commitment to quality make us the ideal partner for your radiolabeling needs in biomolecules. With a focus on precision, efficiency, and minimal biomolecular disruption, we empower researchers to uncover critical insights and drive innovation in drug development and other fields. For additional details about our services or to discuss your requirements, please reach out to us directly.
Please kindly note that our products and services are for research use only.
