Product Focus: Isotope Labeled Ammonium Chloride

Isotope Labeled Ammonium Chloride

Isotope labeling of ammonium chloride is a technique that changes the chemical properties of ammonium chloride by introducing specific isotopes (¹⁵N and/or ²H) into the molecule. Alfa Chemistry supplies high-quality stable isotope labeled ammonium chloride to support your research and needs in the fields of biomolecular NMR, metabolism, metabolomics, proteomics, etc. Our high quality stable isotope labeled ammonium chlorides are available to support your research and needs in the fields of biomolecular NMR, metabolism, metabolomics, proteomics, etc.

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The ammonium ion of ammonium chloride-D₄ contains a lot of deuterium (²H), so this is great for studying hydrogen exchange reactions and other isotope labelling experiments.

• Molecular formula: ND₄Cl
• Molecular weight: 57.52
• Isotopic Enrichment: 98%D

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• Precautions

Ammonium chloride-D₄ has certain hazards, including acute toxicity and eye irritation. It is mainly used in scientific research and is not used as a drug or household medicine.

• Application examples

Ammonium chloride-D₄ can be used as an isotope labeling and tracking chemical reaction source of hydrogen atoms (H).

As a solvent or internal standard in NMR analysis, ammonium chloride-D₄ is also available.

• Preparation method

Ammonium chloride-D₄ can be made by reacting deuterium gas (D₂) with hydrogen chloride (HCl). The end product of the reaction is a mixture of ammonium chloride-D₄ and hydrogen chloride (HCl). Dissolved, purified and dried the mixture, then ammonium chloride-D₄ product can be obtained.

Ammonium chloride-¹⁵N is an abundant source of the stable isotope nitrogen-15, which can be manipulated to examine nitrogen metabolism, protein synthesis and biochemical reactions.

• Molecular formula: ¹⁵NH₄Cl
• Molecular weight: 54.48
• Isotopic Enrichment: ≥98 atom % ¹⁵N

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Application areas

• Biochemistry: The common applications of ammonium chloride-¹⁵N are in the structure and function analysis of proteins. Protein interaction, expression and position can all be measured by biological nuclear magnetic resonance (bio-NMR) imaging.
• Metabolism studies: By adding ammonium chloride-¹⁵N substrate into the experimental system, magnetic resonance imaging (MRS) technology can be used to monitor metabolites at different time points to analyze metabolic pathways.
• Agricultural applications: Ammonium chloride-¹⁵N is a tracer of fertilizer in agriculture to evaluate plant nutrient absorption of nitrogen fertilizer, and calculate best fertilizer application.
• Environmental & Ecology: We can monitor pollution and nitrogen cycles with ammonium chloride-¹⁵N in ecology.

Application examples

• In marine sediment research, ammonium chloride-¹⁵N is used to study the relationship between anaerobic ammonia oxidation and sulfate and iron reduction fuel nitrogen loss.
• In the study of microbial ammonia oxidizing communities, ammonium chloride-¹⁵N is used to analyze the differences in microbial responses to ocean warming.

Ammonium chloride (D₄; ¹⁵N) is dual-labeled with deuterium and nitrogen-15, providing researchers with a powerful tool to study complex chemical and biological systems.

• Molecular formula: ¹⁵ND₄Cl
• Molecular weight: 58.51
• Isotopic Enrichment: 98 atom % D; 98 atom % ¹⁵N

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• Case study

In a study evaluating oral exposure to aerosol constituents of heat-not-burn tobacco products, ammonia concentrations were calculated using linear regression of a 7-point calibration curve prepared using ammonium-¹⁵N,D₄ chloride as an internal standard. [1]