Introduction
Nitrogen-15 (15N) labeled compounds are isotopically enriched molecules where the naturally abundant nitrogen-14 (14N) is selectively replaced with the stable isotope nitrogen-15. By replacing the natural abundance nitrogen atoms with 15N, researchers can easily distinguish between the labeled and unlabeled compounds using techniques such as nuclear magnetic resonance spectroscopy (NMR) or mass spectrometry. This allows for precise measurements of nitrogen-containing molecules and provides valuable information on their behavior and interactions, which play crucial roles in elucidating mechanisms in metabolic pathways, studying nitrogen cycles in ecosystems and others. Therefore, 15N labeled compounds can be used in various fields such as life science, biology, agriculture and environmental science.
Synthesis
The synthesis of 15N labeled compounds can be achieved through various methods, each tailored to incorporate 15N (The primary source of 15N is typically 15N-enriched ammonia or nitrate salts.) into specific molecular frameworks. Here are some common synthesis strategies:
Direct incorporation
- Direct incorporation: One approach involves the direct incorporation of 15N into molecules by using 15N-labeled precursors such as 15NH4Cl or 15NH3 gas in chemical reactions. This method is often used for synthesizing simple 15N labeled compounds like amino acids, nucleotides, and other small organic molecules.
Biosynthetic incorporation
- Biosynthetic incorporation: In biological systems, 15N-labeled compounds can be synthesized through biosynthetic incorporation. For example, for biologically derived compounds like proteins, growing microorganisms (e.g., E. coli) in media containing 15N-labeled sources (like 15NH4Cl) will result in the synthesis of 15N-labeled proteins.
Biosynthetic incorporation
- Isotopic exchange: Isotopic exchange is another method where 14N atoms in a molecule are replaced with 15N atoms using isotopically labeled reagents. This technique is often applied to nitrogen-containing functional groups like amines, amides, and nitriles.
Applications
15N labeled compounds have diverse applications across many scientific disciplines. Here are some notable examples:
Metabolic studies: In biochemistry and molecular biology, 15N labeled amino acids and nucleotides are used as tracers to study metabolic pathways and protein synthesis. By tracking the incorporation of 15N into biomolecules, researchers can elucidate the flow of nitrogen and the dynamics of metabolic networks.
Structural biology: In structural biology, 15N labeling is essential for NMR studies of proteins, nucleic acids, and other biological macromolecules. For examples, 15N labeled proteins are widely used to determine protein structures, study protein-ligand interactions, and investigate dynamic processes at the atomic level.
Ecological and environmental research: In ecology, 15N labeled compounds help in studying nitrogen cycles within ecosystems. For instance, 15N labeled fertilizers are used to track nitrogen uptake and distribution in plants, soil, and water. This helps in understanding nutrient cycling, soil fertility, and environmental pollution.
Agricultural studies: 15N labeled compounds help in studying soil and plant interaction. For example, 15N-labeled fertilizers are also used in agricultural research to study the uptake of nitrogen by plants and its subsequent impact on crop yield and soil health.
What can we offer?
15N labeled compounds are indispensable tools in modern scientific research. As a professional supplier of stable isotope-labeled products, Alfa Chemistry can offer all kinds of 15N labeled compound. Our products with unique properties, enable detailed studies in fields such as metabolic research, environmental science, structural biology, and agricultural studies. Moreover, we ensure rapid and reliable delivery of products to meet your project timelines. If you are unable to find the product you're looking for, we also offer custom synthesis services. Don't hesitate to contact us for any assistance you may require.
Please kindly note that our products and services are for research use only.
