Serra-Compte, Albert, et al. Food and chemical toxicology, 2017, 104, 3-13.
A rapid method based on ultrahigh pressure liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS) was developed for the detection of antibiotics and some of their major metabolites in a variety of seafood matrices. The method allowed the detection and identification of 23 individual compounds (including their four major metabolites).
The recoveries for most compounds were between 30% and 70%, and the method detection limits and quantification limits (MDLs and MQLs) were between 0.01 and 0.31 ng/g dry weight (dw) and 0.02-1.03 ng/g (dw), respectively.
Isotopically labeled compounds used as internal standards included azithromycin-d3, ampicillin-d5, erythromycin-d13, ibuprofen-d3, lincomycin-d3, sulfamethoxazole-d4, ronidazole-d3, ofloxacin-d3, and ciprofloxacin-d8. The internal standard curve was generated using clam extract within a concentration range of 0.01 to 50 ng/g (dw). To reduce matrix interferences and prevent under- or overestimation during quantification, the "internal sample calibration approach" was employed, which involves constructing the calibration curve in the matrix with the addition of isotopically labeled internal standards.
