Li, Hui, et al. Journal of AOAC International, 2011, 94(2), 394-406.
A multi-class/multi-residue method was developed and validated for the determination of 21 veterinary drug residues in shrimp using HPLC/MS/MS, including sulfonamides; tetracyclines; (fluoro)quinolones (e.g., norfloxacin, NOR); and cationic dyes. The method was validated at three levels within the specified linear range for each drug, with accuracies ranging from 77 to 115, and most CV values below 15.
All drugs were quantified within a range no less than 10-fold using matrix-matched standards for linear external standards, with the exception of oxytetracycline, tetracycline, norfloxacin, and ciprofloxacin, which used norfloxacin-d5 as an internal standard.
The right figure shows the chromatograms of all 21 drugs plus NOR-d5 in samples spiked at the L7.5 level. All peaks had good peak shapes and relatively narrow peak widths. Analysis of control shrimp showed that no detectable amounts of target drug residues were found in shrimp available in the market.
