(±)-Nicotine-d4 (pyridine-d4)

Catalog Number	ACM350818698-1
CAS	350818-69-8
Structure
Synonyms	(±)-3-(1-Methyl-2-pyrolidinyl)pyridine
Molecular Weight	166.26
Functional Group	Amines & Amine Salts,Heterocycles
Isotopic Enrichment	98 atom % D
Packaging	0.05 g In Stock 0.1 g In Stock
Shipping Hazards	TOXIC
Stability	Stable if stored under recommended conditions (see section 7 of SDS). After three years, the compound should be re-analyzed for chemical purity before use.
Storage Conditions	Store refrigerated
CAS (Unlabeled)	22083-74-5

Case Study

UHPLC-MS/MS Analysis of Nicotine and Its Active Metabolites for Pharmacokinetic Studies

Liu Y, et al. Biomedical Chromatography, 2019, 33(10), e4634.

The plasma levels of nicotine and its primary metabolite, cotinine, show a strong correlation with their biological effects. A method utilizing UHPLC-MS/MS was developed and validated for the analysis of nicotine and cotinine in mouse plasma.
Chromatographic separation was performed using a BEH HILIC column, with acetonitrile (containing 0.1% formic acid) and 10 mM ammonium formate as the mobile phase. Gradient elution was conducted at a flow rate of 0.4 mL/min, with a total runtime of 3.6 minutes.
The quantitative ion transitions for nicotine, cotinine, and the internal standard nicotine-D4 (IS) were m/z 163.1 > 130.0, m/z 177.1 > 80.0, and m/z 167.1 > 134.0, respectively. The calibration ranges for both nicotine and cotinine extended from 5 to 500 ng/mL, with a lower limit of quantitation set at 5 ng/mL. The intra- and inter-day variability exhibited biases ranging from -4.61% to 12.00%, and imprecision was less than 11.12%.
Recovery, normalized to the IS, was between 90.62% and 98.95% for nicotine and from 89.18% to 101.53% for cotinine. The matrix factors, normalized to the IS, ranged from 106.00% to 116.44% for nicotine and from 100.34% to 109.85% for cotinine. Both nicotine and cotinine demonstrated stability under standard storage conditions.
This validated method has been successfully employed in a pharmacokinetic study in mice to determine the pharmacokinetic parameters for both compounds.

HS-SPME Along with GC-MS Detection of Nicotine in Dried Mushrooms

Jurič A, et al. Analytical methods, 2023, 15(37), 4980-4986.

This work developed a headspace solid phase microextraction (HS-SPME) method and gas chromatography-mass spectrometry detection (GC-MS) to measure nicotine levels in dried mushrooms for food safety.
· Initially, parameters controlling the SPME process such as fiber coating, sample size, extraction temperature and duration, desorption time and salt loading were investigated and optimized. These enhanced conditions were used to confirm a quantitative nicotine analysis by matrix-matched calibration and correction with an isotopically labeled internal standard (Nicotine-d4).
· This resulting method had high linearity (r2 = 0.9994) over the entire concentration range measured (0.025-1 mg kg-1), low detection limit (0.005 mg kg-1) and quantification limit (0.017 mg kg-1) for nicotine, both within the EU food safety limits.
· At the two concentration levels (0.050 and 0.200 mg kg-1), the relative standard deviations of the method were less than 10% (4.5% and 8.5%, respectively), and the 98.2% and 100.3% accuracy was found.
· After that, the optimised technique was used to measure nicotine levels in 18 dried Boletus mushrooms from southeast Europe for sale in the EU. This validated the HS-SPME analytical procedure as being rapid, simple, sensitive, solvent-free, and cost-effective.

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(±)-Nicotine-d4 (pyridine-d4) (350818-69-8)

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