Kang H H, et al. Food Additives & Contaminants: Part A, 2020, 37(10), 1687-1694.
This study reported the development of a GC-MS method for the quantitative analysis of nicotine in mushrooms, based on the QuEChERS extraction method. Recovery was 89.5-92.5%, intra-day precision was 0.32-0.85 %, inter-day precision was 0.73-2.36%, and limits of detection and quantification were 0.38 and 1.15 μg kg-1, respectively. The relative expanded uncertainty was 2.8-4.0%. The analysis method was carried out with 11 mushrooms successfully, containing nicotine values of 0.033-1.713 mg kg-1.
Extraction procedure
· A sample of 1-2 g of homogenized dried mushrooms was placed in a 50 mL tube, to which 10-20 mL of distilled water was added before vortexing.
· It was known that a higher pH correlates with improved recovery. Accordingly, this procedure employed 5 M NaOH to maintain the extract's pH in the range of 10-11. The pH was assessed using pH indicator strips with a scale of 0-14.
· Following this, 0.5 mL of the internal standard solution (nicotine-d3, 2000 μg kg-1) and 10 mL of the extraction solvent (ethyl acetate) were incorporated, and the mixture was vortexed for 1 minute. Subsequently, 6 g of MgSO4 and 1.5 g of NaCl were added, and the mixture was shaken for 15 minutes.
· The mixture was then centrifuged at 1590 g for 5 minutes, and the resulting supernatant was filtered through a 0.45 μm membrane filter to obtain the test solution.
Wiencek J R, et al. American journal of clinical pathology, 2019, 151(5), 516-521.