León, Nuria, et al. Talanta, 2016, 149, 43-52.
A comprehensive strategy was devised that integrates a quantitative method for analyzing 77 prohibited veterinary drugs, mycotoxins, ergot alkaloids, and plant toxins alongside a post-target screening for 425 substances, which include pesticides and environmental contaminants in feed. This approach utilized QuEChERS-based extraction followed by ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS).
The quantitative method underwent validation, yielding recoveries generally between 80% and 120%, and a precision, indicated by a Relative Standard Deviation (RSD), of less than 20%. Full-scan accurate mass data were collected at a resolving power of 50,000 FWHM, with mass accuracy under 5 ppm. The limit of quantification (LOQ) was below 12.5 µg kg-1 for most veterinary drugs and plant toxins and 20 µg kg-1 for ergot alkaloids.
In this study, an internal standard working solution was created, which included ipronidazole-d3, clenbuterol-d9, ractopamine-d5, atenolol-d7, chlorpromazine-d6, ronidazole-d3, dimetridazole-d3, dexamethasone-d4, and chloramphenicol-d5 as internal standards at concentrations of 0.01~1.0 µg ml-1.
