Imidacloprid D4 (imidazolidin-4,4,5,5 D4) 100 µg/mL in Acetone

Catalog Number	ACM1015855750-2
CAS	1015855-75-0
Structure
Synonyms	1H-Imidazol-4,5-d2-2-amine, 1-[(6-chloro-3-pyridinyl)methyl]-4,5-dihydro-4,5-d2-N-nitro-, Imidacloprid D4 (imidazolidin-4,4,5,5 D4), Imidacloprid-d4
IUPAC Name	(NE)-N-[1-[(6-chloropyridin-3-yl)methyl]-4,4,5,5-tetradeuterioimidazolidin-2-ylidene]nitramide
Molecular Weight	259.69
Molecular Formula	C9D4H6ClN5O2
Canonical SMILES	[2H]C1([2H])N\C(=N/[N+](=O)[O-])\N(Cc2ccc(Cl)nc2)C1([2H])[2H]
InChI	InChI=1S/C9H10ClN5O2/c10-8-2-1-7(5-12-8)6-14-4-3-11-9(14)13-15(16)17/h1-2,5H,3-4,6H2,(H,11,13)/i3D2,4D2
Storage	+20 °C
Accurate Mass	259.0774
Format	Single Solution
Shipping Temperature	+20 °C
SIL Type	Deuterium
CAS (Unlabeled)	138261-41-3

Case Study

Imidacloprid D4 for Quantification of Imidacloprid and Its Metabolites

Nimako, Collins, et al. Journal of Chromatography A, 2021, 1652, 462350.

In this work, a sensitive LC-MS/MS method was developed for the simultaneous detection and quantification of imidacloprid (IMI) and its seven metabolites in tissue samples and to determine the biodistribution of IMI compounds in tissues of C57BL/6J male mice. Imidacloprid D4 (IMI-d4) and 6-chloronicotinic acid-13C6 (6-CNA-13C6) were used as internal standards.
Tissue extraction
The optimization process in this study utilized brain tissue from mice that were free of detectable levels of the target chemicals. A 10-15 mg blank brain sample was spiked with 100 μL of an internal standard master mix containing 5 ng of both IMI-d4 and 6-CNA-13C6. The spiked tissue was homogenized in 0.4 mL of 1% formic acid in acetonitrile using a Tissue Lyser. After thorough mixing by vortexing, the sample was centrifuged at 10,000 G for 10 minutes. The resulting supernatant (approximately 0.5 mL) was carefully transferred to a fresh 1.5 mL Eppendorf tube and labeled SP1 (supernatant 1). The tissue residue was then reconstituted in 0.5 mL of methanol, mixed thoroughly by vortexing for 2 minutes, and centrifuged again at 10,000 G for 10 minutes. The second supernatant was similarly collected and labeled SP2 (supernatant 2). Finally, SP1 and SP2 were combined and thoroughly mixed to create the final tissue extract.

Imidacloprid D4 Used in the Multigenerational Toxicity Study of Imidacloprid and Thiacloprid to Spodoptera Candida

van Gestel, Cornelis AM, et al. Ecotoxicology, 2017, 26, 320-328.

Imidacloprid and thiacloprid are neonicotinoid insecticides. This work investigated the toxicity of these two compounds to F. candida after three generations of exposure in a natural LUFA 2.2 standard soil. In the first generation, thiacloprid had a greater effect on reproduction, while imidacloprid was lethally toxic to springtails. In the multigenerational trial, imidacloprid had consistent effects on survival and reproduction in all three generations, while animals exposed to thiacloprid showed significant recovery in the second and third generations.
Imidacloprid D4 for chemical analysis
Soil samples were collected at the beginning of each new generation of exposure, and chemical analysis of imidacloprid and thiacloprid in the test soil was performed. Using stable isotope labeled analogs (imidacloprid-d4 and thiacloprid-d4) as internal standards to prepare soil samples of different concentrations can compensate for potential losses and instrumental variations during sample preparation, thereby improving the accuracy and precision of the analysis.
Imidacloprid and thiacloprid were then quantitatively analyzed using the isotope dilution method with their respective deuterated internal standards. The shift for imidacloprid was 256.1 > 175/209 (quantitative/qualitative), and the shift for imidacloprid-d4 was 260 > 179. For thiacloprid, the shift was 253.1 > 126/90 (quantitative/qualitative), and the shift for thiacloprid-d4 was 257 > 126.

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Imidacloprid D4 (imidazolidin-4,4,5,5 D4) 100 µg/mL in Acetone (1015855-75-0)

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