Progesterone-2,3,4-13C3

Catalog Number	ACM327048873
CAS	327048-87-3
Structure
Synonyms	Progesterone-13C3
Molecular Weight	317.44
Molecular Formula	13C3C18H30O2
Isotopic Enrichment	99 atom % 13C

Case Study

Progesterone-2,3,4-13C3 as an Internal Standard for Steroid Analysis in Human Urine

Takao, Toshifumi, et al. Authorea Preprints, 2023,.

Urine contains both free and bound steroids. This study conducted a thorough quantitative analysis of total and free steroids utilizing UPLC-MS/MS with lithium adduction.
Free steroids were isolated from urine using solid phase extraction (SPE) with 80% acetonitrile. Total steroids were obtained by enzymatically treating urine with a combination of sulfatase and glucosidase, followed by protein precipitation and SPE, as previously described. Both free and total steroids were analyzed using UPLC-MS/MS, with and without the addition of Li+ solution. Quantitative analysis of steroids was performed using two standard curves based on MH+ and [M+Li]+ daughter ion transitions. Progesterone-2,3,4-13C3 (P4-13C3) served as one of the internal standards for the study.
This analytical method detected a total of 29 steroids in human urine, including two newly identified steroids (7-OH P5 and 7-OH DHEA). The accompanying figure presents a bar graph of free and total steroid levels in urine samples from men and women, where P4 represents the measured amount of progesterone.

10 Hormones Including Progesterone Successfully Analyzed from Small Blubber Samples by LC-MS

Wittmaack, Christiana, et al. Frontiers in Marine Science, 2022, 8, 808764.

Understanding stress, reproductive fitness, and health in wild cetaceans is essential for effective conservation and management. This study developed a technique to measure reproductive and stress-related steroid hormones using minimal blubber tissue. Biopsy samples were taken from both free-ranging and stranded gray and fin whales. Steroid hormones were extracted from just 50 mg of blubber using a tailored liquid-liquid extraction method optimized for high-fat content. The extracts were then analyzed by LC-MS to quantify ten hormones: aldosterone, androstenedione, cortisol, cortisone, corticosterone, 17β-estradiol, estrone, 17α-hydroxyprogesterone, progesterone, and testosterone. Isotope-labeled standards, such as progesterone-2,3,4-13C3, supported the analysis.
The method reliably quantified spiked concentrations of all ten hormones with accuracy and precision, confirming its suitability for 50 mg samples from both whale species. In a particular analysis, all endogenous hormones except corticosterone were detected in the gray whale blubber sample, while the fin whale sample lacked detectable levels of aldosterone, corticosterone, estrone, and progesterone.

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Progesterone-2,3,4-13C3 (327048-87-3)

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