Phenol (13C6, 99%)

Catalog NumberACM89059347

CAS Number89059-34-7

CategoryClassification by Compound Category

Molecular Weight	100.07
Purity	98%
Chemical Formula	*C6H5OH
Size	0.1 g
CAS (Unlabeled)	108-95-2

Case Study

Mapping Anaerobic Biodegradation Pathways with Phenol-13C6

Hoyos-Hernandez, Carolina, et al. 13th World Congress on Anaerobic Digestion: Recovering (bio) Resources for the World. 2013.

Understanding the microbial drivers of differential phenol biodegradation kinetics under anaerobic conditions required precise tracking of phenol mineralization pathways and correlating degradation rates with specific bacterial populations. In this work,
phenol-13C6 served as the critical isotopic tracer to quantify phenol depletion kinetics in real-time, confirm complete mineralization to 13CO2/13CH4, and enable microbial population analysis via DNA extraction and pyrosequencing analysis correlated with degradation rates.
Key Findings:
Using Phenol-13C6 revealed key biodegradation mechanisms:
· Dual Kinetic Modes: Identified first-order versus lag-phase kinetics in both mesophilic (35°C) and thermophilic (55°C) municipal solid waste microcosms.
· Microbial Community Linkage: Pyrosequencing linked accelerated kinetics to Clostridium (mesophilic) and Anaerobranca (thermophilic), with relative abundance dictating degradation rates.
· Pathway Differentiation: Faster mesophilic degradation proceeded via benzoic acid, while thermophilic Anaerobranca potentially enhanced bioavailability via cyclodextrin synthesis.
· Mineralization Confirmation: Isotopic tracking verified complete conversion to CO2/CH4.

Tracking Phenol-13C6 Dynamics in Activated Sludge SIP Research

Manefield, Mike, et al. Journal of microbiological methods 69.2 (2007): 340-344.

This case study analyzed the metabolic fate of Phenol-13C6 in an activated sludge stable isotope probing (SIP) experiment, tracking its distribution and incorporation into microbial biomarkers.
Methodology: Researchers introduced a single pulse of Phenol-13C6 and meticulously tracked the subsequent distribution of the 13C label across different components of the system over time, using SIP principles with nucleic acids (DNA and RNA) as the targeted biomarkers.
Key Findings:
· Within 100 minutes of introduction, most Phenol-13C6 was metabolized: 49% incorporated into biomass, 6% respired as 13CO2, and the remainder adsorbed to solids. Less than 1% of supplied 13C entered nucleic acids, with RNA labeling 6.5× faster than DNA. Crucially, nucleic acid 13C content continued rising after phenol depletion, indicating rapid cross-feeding of metabolic derivatives.
· Phenol-13C6 effectively traces carbon flow but requires strategic sampling: Early sampling captures primary phenol utilizers before cross-feeding dominates, while extended sampling tracks community-wide label propagation. RNA-SIP's faster labeling offers advantages for detecting active degraders.

Please kindly note that our products and services are for research use only.

Online Inquiry



mg g kg ml L

Phenol (13C6, 99%) (89059-34-7)

SUBMIT