Deuteration Rate Determination - Isotope Science / Alfa Chemistry

Our Service: Comprehensive Deuteration Rate Solutions

Deuteration Rate Determination

The deuteration rate serves as a fundamental metric for assessing isotopic incorporation in drug molecules. Our specialized service encompasses precise quantification of deuterium content using cutting-edge analytical techniques such as nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. These methodologies enable the accurate determination of isotopic distribution, ensuring compliance with regulatory standards and facilitating informed decision-making during drug development.

Method Development and Validation of Deuteration Rate Determination

In addition to routine deuteration rate testing, we offer method development and validation services of deuteration rate determination for clients requiring tailored solutions. This is especially important when working with novel or complex drug molecules where standard methods may not be applicable. Our service can design and validate new deuteration rate determination analytical techniques for your specific needs.

Our Test Methods

NMR Spectroscopy

Mass Spectrometry (MS)

Innovative Techniques

Test Methods

NMR Spectroscopy

NMR spectroscopy leverages the distinct properties of hydrogen (H) and deuterium (D) in hydrogen-spectrum NMR testing. By adjusting specific pulse sequences, it is possible to generate signals for hydrogen while suppressing signals for deuterium. Using either internal or external standards, this method accurately determines the substitution degree of deuterium isotopes. NMR offers several advantages, including high sensitivity, excellent reproducibility, and exceptional accuracy, making it a preferred choice for precise

Mass Spectrometry (MS)

At Alfa Chemistry, mass spectrometry is another critical tool for determining the deuteration rate. Mass spectrometry allows us to measure the exact mass of a molecule and identify the isotopic distribution, providing highly accurate deuteration rate results. By comparing the mass-to-charge ratios of the deuterated and non-deuterated versions of the molecule, we can precisely quantify the degree of deuteration. This method is especially useful for large or complex molecules, offering superior sensitivity and specificity.

Innovative Techniques

In addition, we developed a novel quantitative method by using relative 1H NMR without the need for internal or external standards. This technique uses mixed deuterated reagents as solvents to alter the coupling of active hydrogen with residual methyl 1H in deuterium-labeled molecules. By converting the methyl 1H signal into a single peak, this approach enhances sensitivity and enables highly accurate deuteration rate analysis.

Why Partner with Us?

Advanced Instrumentation: We use the latest NMR and MS systems from leading manufacturers to ensure that we can deliver the highest quality data with exceptional accuracy and precision.

Extensive Experience: Our team brings years of experience in deuterated drug analysis. This extensive experience allows us to solve any challenge.

Comprehensive Data Analysis: We provide clients with detailed reports that include both raw data and expert analysis, which can make informed decisions about the next steps in their drug development process.

With advanced analytical techniques, extensive experience, and a commitment to regulatory compliance, Alfa Chemistry provides reliable and accurate deuteration rate analysis of deuterated drug candidates. For additional details about our services or to discuss your requirements for deuteration rate determination, please reach out to us directly.

Please kindly note that our products and services are for research use only.

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